Sustainable valorisation of organic urban wastes : insights from African case studies

Understanding the problems and potentials of the organic waste stream is perhaps the single most important step that city authorities in Africa could take in moving towards sustainable, affordable, effective and efficient waste management. This publication presents four examples of recent attempts to manage organic waste sustainably in the African context. The participants in the ‘Nairobi organic urban waste’ project have structured this case exercise in order to use the case studies as object lessons, to harvest genuine insights into the feasibility of a variety of ways to successfully and sustainably valorise urban organic waste streams. Three contemporary case examples of compost production are presented. These include composting by a community-based organisation in the Kenyan private sector and by a public-private partnership in Malawi. In all three cases, the project and case study focus is on the relations between city waste and the agricultural supply chain. A fourth case study describes the technical and economic potential to produce and use biogas from urban organic waste

Comparison of proteomic responses as global approach to antibiotic mechanism of action elucidation

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. New antibiotics are urgently needed to address the mounting resistance challenge. In early drug discovery, one of the bottlenecks is the elucidation of targets and mechanisms. To accelerate antibiotic research, we provide a proteomic approach for the rapid classification of compounds into those with precedented and unprecedented modes of action. We established a proteomic response library of Bacillus subtilis covering 91 antibiotics and comparator compounds, and a mathematical approach was developed to aid data analysis. Comparison of proteomic responses (CoPR) allows the rapid identification of antibiotics with dual mechanisms of action as shown for atypical tetracyclines. It also aids in generating hypotheses on mechanisms of action as presented for salvarsan (arsphenamine) and the antirheumatic agent auranofin, which is under consideration for repurposing. Proteomic profiling also provides insights into the impact of antibiotics on bacterial physiology through analysis of marker proteins indicative of the impairment of cellular processes and structures. As demonstrated for trans-translation, a promising target not yet exploited clinically, proteomic profiling supports chemical biology approaches to investigating bacterial physiology